Shiitake Liquid Culture

Shiitake (lentinula edodes) liquid culture syringe and inoculation kit.

One syringe should be enough to inoculate 2-3 jars or bags of grain spawn.( Use 2ml-5mL per container, depending on size). See instructions below for more information.

If the syringe is not being used within a couple of weeks, store in refrigerator( not freezer). You can keep it up to 6 months or more this way. Although we recommend you use it as soon as possible to ensure vigor of they mycelium. 

Kit Content:

- Sterile 10ml/cc liquid culture syringe

- Medical grade sanitization wipes

- 18g needle for injection into spawn or substrate

Instructions for Use:

1. In a sterile environment (flow hood, still-air box, etc.) inject approximately 2-5mL of liquid culture into a sterile spawn bag or jar, the more LC you use, the faster the colonization.

2. Store the grain spawn somewhere dark or without significant direct sunlight, at optimum temperature of 70-76 F ( 20 C- 25 C) and wait approx. 7-10 days for the entire bag or jar to colonize with mycelium.

3. Once your grain spawn is fully colonized, you can now transfer to other grain jars to make more spawn, or inoculate bulk substrate of choice.